Methods


Bacillus cereus count*

Method References


FDA BAM 8th Edition, Chapter 14, 2001

Sample Size


25g

Estimated Turn Around Time


48 hours. If presumptive colonies, additional 48 hours for confirmation.

Reporting Unit


cfu/mL
cfu/g
cfu/sampled area

Limit of Detection


Standard *<10 cfu

Method Description


Samples are prepared and dilutions made. Plated on selective media and incubated based on product type. Plates are then read, and results generated based on countable range.

Additional Information


Bacillus cereus is an aerobic spore-forming bacterium that is commonly found in soil, on vegetables, and in many raw and processed foods.
 
The MYP medium is used to help differentiate B. cereus from other bacteria based on the organism’s resistance to polymyxin, lack of mannitol fermentation and presence of lecithinase.  The absence of mannitol results in the agar changing to a pink-red color, and the presence of lecithinase creates the zone of precipitate surrounding the colony.

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