Clostridium perfringens (C. perfringens) Pour

Method References

ISO 7937: 2004 – Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of Clostridium perfringens
VITEK System for Identifications

Sample Size


Estimated Turn Around Time

24 hours (If presumptive colonies, additional 48-50 hours)

Reporting Unit

cfu/sampled area

Method Description

Samples are prepared and dilutions are made. Incubated anaerobically and removed after 24 hours. Plates are then counted, and colonies followed up if presumptive.

Additional Information

Clostridium perfringens (C. perfringens) is a spore-forming gram-positive bacterium that is found in many environmental sources as well as in the intestines of humans and animals. It forms black colonies on agar. The black precipitate is caused by the reduction of sulfite to sulfide

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