Samples are prepared and dilutions are made. Incubated anaerobically and removed after 24 hours. Plates are then counted, and colonies followed up if presumptive.
Clostridium perfringens (C. perfringens) is a spore-forming gram-positive bacterium that is found in many environmental sources as well as in the intestines of humans and animals. It forms black colonies on agar. The black precipitate is caused by the reduction of sulfite to sulfide