Methods


Cronobacter sakazakii ISO

Method References


ISO 22964-Microbiology of the food chain-Horizontal method for the detection of Cronobacter ssp. 2017-04

Sample Size


50g

Estimated Turn Around Time


72 hours (If presumptive, confirmation is an additional 2 days)

Reporting Unit


Positive or Negative /g

Limit of Detection


Result is based on colony morphology and Positive/Negative is given.

Method Description


Samples are prepared and incubated for 24 hours. Enrichment is added and placed in a tube. Incubated for an additional 24 hours. Sample is then plated on selective media and incubated based on product type. Plates are then read and results generated based on colony morphology.

Additional Information


Cronobacter consists of six species and they are Cronobacter sakazakii, C. turicensis, C. malonaticus, C. muytjensii, C. dublinensis and C. genomospecies I. All species have been linked to clinical cases of infection and therefore all species should be considered pathogenic.

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