Fecal Coliform Three Tube MPN (Most Probable Number)
Fecal Coliform Five Tube MPN for Sludge Samples
Fecal Coliform Five Tube MPN for Water and Wastewater
Fecal Coliform Ten Tube MPN for Water

Method References

Standard Methods for the Examination of Water and Wastewater
AOAC Official Method 966.24 Coliform Group and E. coli
FDA BAM Chapter 4: Enumeration of Escherichia coli and the Coliform Bacteria

Sample Size


Estimated Turn Around Time

48 hours (If presumptive, additional 24-48 hours)

Reporting Unit

MPN/grams of TS

Method Description

Samples are prepared and dilutions made in MPN tubes and incubated based on method specifications. MPN tubes are then read and any tubes that produce gassing or effervescence are carried on for confirmation. Results generated based on number of positive tubes in the confirmation.

Additional Information

Fecal coliforms are one type of coliform bacteria that are found mainly in animal digestive tracts and feces. Fecal coliforms are a more specific indicator of fecal contamination of water. A confirmed positive test for fecal coliforms or E. coli means you need to take action as advised by your water system
These procedures listed above provide the most probable number (MPN) result to indicate the amount of fecal coliform present in a sample.

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