Methods


Mesophilic Aerobic Sporeformer Count
Mesophilic Anaerobic Sporeformer MPN (Most Probable Number)

Method References


Standard Methods for the Examination of Dairy Products
Compendium of Methods for the Microbiological Examination of Foods
US FDA Bacteriological Analytical Manual

Sample Size


50g

Estimated Turn Around Time


Aerobic Plate: 48 hours
Anaerobic MPN: 7 days

Reporting Unit


Plate: cfu/mL, or cfu/g
MPN: MPN/mL or MPN/g

Limit of Detection


N/A

Method Description


Plate: Samples are prepared and dilutions made. The samples are then plated on media and incubated based on method specifications. After incubation plates are read and results generated based on countable range.
 
MPN: Samples are prepared and 3 dilutions (3 tubes each) are made. The tubes are incubated per method specifications, checked at 2 days and 4 days, and a final read after 7 days.

Additional Information


A mesophile is an organism that grows best in moderate temperature, neither too hot nor too cold, with an optimum growth range from 20 to 45 °C (68 to 113 °F. Spore-forming bacteria is known to be able to survive extreme processing conditions.
 
Spore-forming bacteria of the species Bacillus are common spoilage organisms that can survive pasteurization. Spore forming bacteria are known to have an intrinsically higher resistance to sanitizers.
 
These procedures listed above enumerate/provide the most probable number (MPN) result to indicate the amount of mesophilic spore former organisms present in a sample.

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