Pseudomonas Spread Plate Count
Pseudomonas Membrane Filtration Count
Pseudomonas aeruginosa USP 62*

Method References

Standard Methods for the Examination of Dairy Products
Compendium of Methods for the Microbiological Examination of Foods
U.S. Pharmacopeia Chapter <62> Microbial Examination of Nonsterile Products: Test for Specific Microorganisms

Sample Size

Membrane filtration: 100mL

Estimated Turn Around Time

48-72 hours

Reporting Unit

Spread Plate: cfu/mL or cfu/g
USP testing: Presumptive or Negative /10g
Membrane Filtration: cfu/100mL

Method Description

Samples are prepared and dilutions made. Plated on media and incubated based on product type. Plates are then read and results generated based on countable range or presence/absence of typical growth.
USP samples are enriched then plated and incubated. Plates are then read and results generated based on presence/absence of typical growth.

Additional Information

Pseudomonas species can cause food spoilage in specific food groups such as milk and dairy products, meat, fish, water, fruit, and vegetables. Most Pseudomonas species do not constitute a serious risk to public health. However, Pseudomonas aeruginosa is an opportunistic pathogen and can cause infection in certain individuals.
These procedures detect/enumerate pseudomonas in a sample using a spread plate, membrane filtration or enrichment method.

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