Methods


Pseudomonas Spread Plate Count
Pseudomonas Membrane Filtration Count
Pseudomonas aeruginosa USP 62*

Method References


Standard Methods for the Examination of Dairy Products
Compendium of Methods for the Microbiological Examination of Foods
U.S. Pharmacopeia Chapter <62> Microbial Examination of Nonsterile Products: Test for Specific Microorganisms

Sample Size


50g
Membrane filtration: 100mL

Estimated Turn Around Time


48-72 hours

Reporting Unit


Spread Plate: cfu/mL or cfu/g
 
USP testing: Presumptive or Negative /10g
 
Membrane Filtration: cfu/100mL

Limit of Detection


N/A

Method Description


Samples are prepared and dilutions made. Plated on media and incubated based on product type. Plates are then read and results generated based on countable range or presence/absence of typical growth.
 
USP samples are enriched then plated and incubated. Plates are then read and results generated based on presence/absence of typical growth.

Additional Information


Pseudomonas species can cause food spoilage in specific food groups such as milk and dairy products, meat, fish, water, fruit, and vegetables. Most Pseudomonas species do not constitute a serious risk to public health. However, Pseudomonas aeruginosa is an opportunistic pathogen and can cause infection in certain individuals.
 
These procedures detect/enumerate pseudomonas in a sample using a spread plate, membrane filtration or enrichment method.

Looking for More Info?

Analytes with * are on Cherney's Scope of Accreditation.
 
(R) - Rush services available