Methods


Coagulase Positive Staphylococcus Spread Plate*
Staphylococcus Express Count Petrifilm*
Staphylococcus species Spread Plate*
Coagulase Positive Staphylococcus MPN (Most Probable Number)

Method References


Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) ISO 6888
Compendium of Methods for the Microbiological Examination of Foods
US FDA Bacteriological Analytical Manual
3M™ Petrifilm Staph Express Count Plate Interpretation Guide

Sample Size


25g

Estimated Turn Around Time


Petrifilm: 24 hours
 
Spread Plate: 48 hours
 
MPN: 96 hours

Reporting Unit


Spread Plate/Petrifilm: cfu/mL, cfu/g, cfu/sampled area
 
MPN: MPN/g

Limit of Detection


N/A

Method Description


Samples are prepared and dilutions made. The samples are then plated on media and incubated based on method specifications. After incubation plates are read and results generated based on countable range.

Additional Information


Staphylococcus aureus is an opportunistic pathogen that can cause a wide spectrum of infections, from superficial skin infections to severe illness. When present in food, staph aureus could contaminate the product with a toxin, causing staphylococcal food-borne disease.
 
This procedure enumerates the number of staphylococcus in a sample using a spread plate, petrifilm or MPN (Most Probable Number) method.

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